Relia Tech/Porcine ECGF (Crude Extract, cell culture grade) + Heparin/5x6 mg/300-092H-5,Relia Tech,,Relia Tech,reliatech,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Relia Tech/Porcine ECGF (Crude Extract, cell culture grade) + Heparin/5x6 mg/300-092H-5

产品编号：300-092H-5

市场价：¥4500.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞培养>添加物>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$225.00

品牌： Relia Tech

公司：Receptor Ligand Technologies GmbH

公司分类：

商品介绍

Cat-Nr.	300-092H-5
Size	5x6 mg
Price	225 €
Source	Swine
Formulation	lyophilized (freeze-dried)
Purification	Crude extract
Biological Activity	The optimal concentration for the cultivation of human umbilical vein endothelial cells (HUVEC) with heparin (30 µg/ml) is about 12 µg/ml.
Species Reactivity	human cells, mouse cells, rat cells, cattle cells, pig cells
Buffer	water
Reconstitution	Reconstitute the contents of the vial in 2 ml of prewarmed (37°C) sterile PBS or water. Gently rotate the vial until the contents are dissolved. This stock solution may be further diluted in sterile tissue culture media to obtain the desired working concentrations. It is recommended that medium containing diluted product is aseptically filtered prior to use.
Stability and Storage	Also stable at 4°C for several weeks it is recommended to store the product below 0°C. After reconstitution the product should be stored in aliquots at -20°C to -70°C.
Application Remarks	Can be used for endothelial cell cultivation.
Synonyms	Endothelial cell growth factor (ECGF); Endothelial cell growth supplement (ECGS)
Description	Endothelial cell growth factor (ECGF) is an extract of bovine brain containing growth promoting factors for vascular endothelial cells of mammalian origin. ECGF has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production. Endothelial cell growth factor is prepared using a modification of the method of Maciag, et al. (1979) lyophilized from a sterile solution containing NaCl and streptomycin sulfate. Endothelial cells from human umbilical vein (HUVEC) can be established as primary cultures by traditional methods. The serial propagation of these cells has proved to be difficult. The long-term propagation of these cells in vitro can be achieved with an extract prepared from porcine brain. The introduction of a fibronectin or collagen matrix to the cell culture system allows to cultivate endothelial cells at clonal densities. With ECGF, the FCS requirement can be reduced. Heparin potentiates the mitogenic activity of crude preparations of ECGF. ECGF has also been reported to eliminate the need for feeder cells in the clonal growth of hybridomas and other cell types.

品牌介绍

ReliaTech

成立于1999年。除其他研究产品外，公司还开发、生产和销售用于血管生成和淋巴管生成研究的高质量研究试剂。

ReliaTech GmbH (short for Receptor Ligand Technologies GmbH) is a post-genomic German biotechnology company founded in 1999. Besides other research products the company develops, produces and sells high quality research reagents for angiogenic and lymphangiogenic research.

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